HEPES is a zwitterion buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures (Molecular Biology Grade)
| Synonym | 4-(2-Hydroxyethyl)-1-piperazine-1-ethanesulfonic Acid, N-(2-Hydroxyethyl)-piperazine-N’-2-ethanesulfonic Acid |
| State of matter | Solid |
| useful pH range | pH 6.8 – 8.2 |
| pKa (20°C) | 7.55 |
| Melting point | 210 – 215°C |
| Formula | C8H18N2O4S |
| M | 238.31 g/mol |
| CAS-No.: | 7365-45-9 |
| HS-No.: | 29335995 |
| EC-No.: | 230-907-9 |
| Storage: | RT |
| LGK: | 10 – 13 |
| Disposal: | 3 |
| WGK: | 1 |
| Specification | |
| DNases/RNases/Proteases | not detectable |
| Assay |
min. 99.5 % |
| pH (1 %; H2O) |
4.7 – 6.0 |
| Water |
max. 0.25 % |
| Chloride | max. 0.005 % |
| Sulfate | max. 0.005 % |
| As | max. 0.0001 % |
| Fe | max. 0.001 % |
| Pb | max. 0.001 % |
| A (1 cm/0.1 M HPLC water) 260 nm/
280 nm |
max. 0.1 max. 0.05 |
| Product | Description |
| CE9171 | HEPES
100 g |
| CE9172 | HEPES
500 g |
| CE9173 | HEPES
1000 g |
Datasheet
Stability Test Report
Material Safety Datasheet
