Description:
Excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide ge (Molecular Biology Grade)
| Synonym | Cleland’s Reagent, 2,3-Dihydroxybutane-1,4-dithiol, 1,4-Dithio-DL-threit(ol) |
| State of matter | Solid |
| Solubility (20°C) | 1500 g/L (H2O) |
| Melting point |
42 – 44°C |
| Formula | C4H10O2S2 |
| M | 154.25 g/mol |
| CAS-No.: | 3483-12-3 |
| EC-No.: | 222-468-7 |
| Storage: | 2 – 8°C, under inert gas |
| Shipment: | ambient temperature |
| LGK: | 10 – 13 |
| Disposal: | 3 |
| Hazard pictogram(s) |  |
| Hazard statement(s) | H302-H315-H319 |
| Precautionary statement(s) | P302+P350-P305+P351+P338 |
| Signal word | Warning |
| WGK: | 1 |
| Attention:
Material is highly hygroskopic! Storage at 2°-8°C under inert gas! Open only under inert gas! |
| Specification | |
| DNases/RNases/Proteases | not detectable |
| Assay (.) | min. 99.5 % |
| pH (0.1 M; H2O; 20°C) | 4.0 – 6.0 |
| DTT (oxidized) | max. 0.5 % |
| Loss on drying | max. 0.5 % |
| A (1 cm/0.02 M in H2O) | |
| 283 nm | max. 0.05 |
| Product | Description |
| CE9131 | DTT
5 g |
| CE9132 | DTT
10 g |
| CE9133 | DTT
25 g |
Datasheet
Stability Test Report
Material Safety Datasheet
